Anti Acetylcholinesterase (human brain, AChE)
mouse monoclonal antibody
Cat.No. HYB 111-05
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa (1,2).
Brain acetylcholinesterase, human, purified and detergent solubilized (1).
HYB 111-05 has specificity for human acetylcholinesterase. No cross-reaction is seen with human BtChE, or with AChE from flounder or Torpedo marmorata.
Not determined
HYB 111-05 reacts with both G2-and G4-AChE (native detergent soluble) in ELISA and EAIA using the antibody as capture antibody. HYB 111-05 reacts 17 times stronger with human brain AChE than with human erythrocyte AChE.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Sorensen K, Gentinetta R, Brodbeck U (1982) An amphiphile-dependent form of human brain caudate nucleus acetylcholinesterase: purification and properties. J Neurochem 39:1050-1060.
2. Massoulie J, Bon S (1982) The molecular forms of cholinesterase and acetylcholinesterase in vertebrates. Annu Rev Neurosci 5:57-106.
3. Liao J, Boschetti N, Mortensen V, Jensen SP, Koch C, Norgaard-Pedersen B, Brodbeck U (1994) Characterization of salt-soluble forms of acetylcholinesterase from bovine brain. J Neurochem 63:1446-1453.
4. Liao J, Mortensen V, Noregaard-Pedersen B, Koch C, Brodbeck U (1993) Monoclonal antibodies against brain acetylcholinesterases which recognize the subunits bearing the hydrophobic anchor. Eur J Biochem 215:333-340.
5. Aziz-Aloya R, Sternfeld M, Soreq H (1993) Promoter elements and alternative splicing in the human ACHE gene. Prog Brain Res 98:147-153.
6. Norgaard-Pedersen B, Hangaard J, Bjerrum OJ (1983) Quantitative enzyme antigen immunoassay of acetylcholinesterase in amniotic fluid. Clin Chem 29:1061-1064.
